Well, I took the plunge! I got the Highlander Hybrid AWD Limited w/ mats and nav for $38599, before taxes and fees.
I drove ~45 miles last night, mostly highway (70 MPH), and got 25.6 MPG! I'm VERY impressed, especially after reading about the lower MPG in the winter. I think the screen said it was 42 degrees outside.
The only complaint I have is with the homelink system, it's not compatible with all garage door openers. At least, I couldn't get it to work, but I'll try again later.
BTW, did anybody's dealer make them sign a piece of paper saying you agree not to install any accessories on your vehicle?