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Heeyyy, Bay Area mechanic here (Santa Clara) that works on a lot of RX300/RX330. Weak point will be the transmission, that U140F is not great (have done two replacements so far), they didn't get most of it right until the U151 in the RX330. And those were daily drivers, you put yours through more than they ever did.
Install an auxiliary cooler and some sort of filter; I used to install Magnefines (still do, actually) but am trying a remote can-style oil filter this time. I would also consider installing a cold-weather bypass if you spend a lot of time in Tahoe. It won't do much in inland Cali weather, but will keep the trans from experiencing cold fluid-shock in snowy climes.
On a U140E/F, if you hear whining in 1st/2nd, it's on its way out (might be 5K, might be 50K depending on abuse). Synthetic fluid is a good idea; I run Valvoline MaxLife Dex/Merc in all the ones I service, shifts better, runs a bit cooler, and trans are still shifting fine when I recommend changes (every 25-30K on the U140, 40-50K on U151). In fact, the MaxLife works better than the T-IV I used to put in ES300 and RX300 where we were running 15K intervals to try and stave off trans failure...
00 Camry 5S-FE 178k BLOWN MOTOR/TRANS
01 ES300 147k NEEDS TRANS WORK
01 Insight 137k BROKEN CAMSHAFT
02 Insight 178k DC-DC BELLY-UP
08 STS-V 67k 570 RWHP!
01 Viggen 112k 400 FWHP